Phylogenetics Tree Report

Phylogenetics Tree Report

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Phylogenetics Tree Report

Introduction

Phylogenic tree can be described as a diagram or a tree that shows the relationships of the various different species based on their physical or genetic characteristics. The phylogenic tree establishes that the each species no matter how different they are, they have a certain relationship among them. The use of proteins is one of the ways the phylogenetics tree is used to establish relationships between the different species. Finally, the tree shows that the different species at one point they originated in the same place. For instance, the phylogenetics tree can be used to prove that different types of fish species have similarities derived from where they came from.

Method

In order to draw a phylogenetics tree, proteins from the different fish species is needed. The method that is going to be to extract protein from these species is referred to as the polycrylimide gel electrophoresis. In this method, proteins are separated from the species by the use of charge or size. This process works like a sieve. This is because the protein molecules are too big to pass the gel pores. When they are charged, they are unable to move through the gel thus they be separated from the species. Therefore, it can be noticed that the purposes of the polycrylimide is to separate the protein molecules through electrical charge, and at the same time, measure the size of the protein molecules. It should be noted that, the electrical charges are introduced to help the proteins migrate into the gel for the purposes of measuring their size to be used in the experiment.

Results

After measuring the distances of protein migration in the fish species, it was found that Spotty-fish had proteins of 28.6 kD, 32.5 kD, and 21.7 kD. Happy fish had proteins of 32.5kD, 31.5kD, 25.6kD, 20.2kD and 21.7kD. Stripy fish had proteins of 31.5kD, 28.6kD, 21.6kD, 21.2kD and 20.2kD. Finally, Gripy fish had proteins of 31.5kD, 31.0kD, 25.6kD, 21.7kD, 21.2kD and 20.2kD. All this values indicate the different types of proteins that were found in the different species of fish that were being used for the experiment.

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